Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing
Merkert S, Haase A, Dahlmann J, Göhring G, Waqas F, Pessler F, Martin U, Olmer R
Published in
Stem cell research: Volume 69, Page 103090
Abstract
The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway.
Open in PubMedAuthors
Prof. Dr. Frank Pessler
Research Group Leader
Dr. Fakhar Waqas
Postdoctoral Researcher | Project Leader “Host-directed Antiviral Compounds”