2023
Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing
Merkert S, Haase A, Dahlmann J, Göhring G, Waqas F, Pessler F, Martin U, Olmer R
Erschienen in
Stem Cell Research: Volume 69, Page 103090
Abstract
The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway.
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