Longitudinal plasma proteomic signatures of elite and viremic spontaneous HIV controllers
Vadaq N, Groenendijk A, Dos Santos J, Mehta K, Wit F, Vos W, Blaauw M, van Eekeren L, Lambrechts L, Rutsaert S, Nelwan E, Xu C, Joosten L, de Mast Q, Matzaraki V, van Lunzen J, Rokx C, Verbon A, Netea M, Vandekerckhove L, van der Ven A
Erschienen in
Nature Communications, Page 10.1038/s41467-025-67939-3
Abstract
A small percentage of people living with HIV (PLHIV) spontaneously regulate viral replication without suppressive antiretroviral treatment (ART) and are categorized into 'elite controllers' (EC, HIV-RNA < 50 c/mL) and 'viremic controllers' (VC, HIV-RNA between 50-10,000 c/ml). Some EC and VC may lose controller status in time. Here we provide extensive plasma proteomics to identify biomarkers and pathways related to spontaneous viral control and its long-term preservation among 36 EC and 147 VC (discovery) and 14 EC and 5 VC (validation). VC exhibited higher concentrations of CRTAM, LY9, and CD6 and lower concentrations of VAT1 compared to EC in both the discovery and validation cohort. Longitudinal analysis of pre- and post-ART samples (median follow-up: 5.3 years) revealed downregulation of various immune-related proteins in both EC and VC. Over a 17-year follow-up period, loss of viral control occurred in 31% of VC and 3% of EC. T-cell associated proteins (CRTAM, LY9, CD6), along with ICAM3, SH2D1A, C1QL2, and CNGB3, predicted loss of viral control years before its occurrence. Markers of chronic immune activation (sPD-L1, sCD25, IL-10, TGF-β, IFN-γ, and TNF-α) and systemic inflammation (TNF, IL-1β, IL-6, and sCD14) were not predictive. Our findings underscore the dynamic interplay between T cell function and viral replication in maintaining HIV control and identify key biomarkers that predict viral load surges.
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